Tel: +44 (0) 1904 406082

Procedure for Seralite® – FLC ELISA

The following describes the procedure for Seralite® – FLC ELISA (For Research Use Only) kappa and lambda free light chain assays.

Prepare reagents and dilute samples as required with sample diluent buffer.

  • Add 100µL calibrator, control or sample to the microplate well.
    • Incubate for 60 minutes at room temperature.

     

  • Wash the plate x 3 with wash buffer
    • Add 100µL of prepared Kappa-HRP or Lambda-HRP detection antibody to the appropriate plate

     

  • Incubate for 30 mins at room temperature.
    • Wash the plate x 3 with wash buffer

     

  • Add 100µL of substrate solution to each well.
    • Incubate for 30 mins at room temperature in the dark.

     

  • Add 50µL of stop solution to each well.
    • Read absorbance at 450nm.