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Seralite® – FLC* utilises highly specific, anti-K and anti-λ monoclonal antibodies (mAbs) which are conjugated to gold particles and incorporated into a simple to use lateral flow device.

The serum is added to the sample application buffer provided with the kit and then added to the sample port. The sample flows through the conjugate pad containing the gold nanoparticles coupled to mAbs.

Sample, and the mAb gold conjugate flow through a membrane containing two test lines, one pre-coated with purified Κ FLC and the other with purified λ FLC. A third line made up of immobilised biotin acts as a control line. Inhibition of mAb-gold binding to the test lines indicates the presence of FLC in the sample.

Schematic view of free light chain test, Seralite- FLC

Click to enlarge

After ~10 minutes the sample is analysed on a CE marked and FDA registered lateral flow assay reader (ADxLR5®) which reads the results of the device, quantifies the kappa and lambda FLC and calculates the ratio of Κ/λ FLC.

An abnormal K/λ ratio indicates monoclonal FLC production.

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*Not available for sale in the USA