There are several important elements to consider when developing a quantitative lateral flow assay. In the fifth edition of our video series, Abingdon Health Nanos, Assay Development Manager Meena Shemar outlines 3 factors to consider when making a quantitative lateral flow assay.
The transcript for the video, ‘3 factors to consider when making a quantitative lateral flow assay’.
"Firstly, a calibration curve will be required. For this, it is important to source suitable calibrator standards that cover the desired assay measuring range.”
“Secondly, selecting suitable raw materials, such as the label and antibodies, both can have a profound effect on the assay performance. For example, high-affinity antibodies are capable of detecting much lower levels of the detection analyte. The size of the particle will also have a profound impact on the assay’s sensitivity.”
“Thirdly, choosing a lateral flow reader capable of detecting the label, that is precise and has a wide dynamic range.”