Tel: +44 (0) 1904 406082

ELISA based free light chain assay for use with biological fluids

Utilising well characterised, highly specific, highly sensitive monoclonal antibodies, Seralite®– FLC ELISA (For Research Use Only) provides a robust and easy to use option for free light chain testing in biological fluids, utilising the same monoclonal antibodies used in our lateral flow method, Seralite®– FLC. The ELISA is a sequential sandwich assay format.

Seralite®– FLC ELISA is a 96 well format, 2 plate kit (1 x FLC-kappa, 1 x FLC-lambda) and can be used by either manual methods or using automated ELISA platforms.   Capture antibodies are supplied coated to 96 well (12 x 8) microplates with signal generated by antibodies labelled with horseradish peroxidase.

Cost Efficient

  • Cost effective, transparent kit pricing
  • No antigen excess reducing the need for repeat testing
  • Wide dynamic measuring ranges

Easy to use

  • ELISA format
  • Well understood
  • Easy to automate
  • High throughput
  • Scalable to requirements

Proven Technology

Confidence in Results

  • No Antigen Excess
  • Excellent lot to lot reproducibility
  • Highly specific, monoclonal antibodies in sequential sandwich assay format
  • Well characterised antibodies

For more information using Seralite®– FLC ELISA for free light chain testing please contact us on +44 (0) 1904 406080 or


  1. Fischer C, et al Kappa free light chains in cerebrospinal fluid as markers of intrathecal immunoglobulin synthesis. Clin Chem 2004;50:1809-13
  2. Presslauer S, et al Elevated levels of kappa free light chains in CSF support the diagnosis of multiple sclerosis. J Neurol 2008;255:1508-14
  3. Presslauer S, et al Kappa Free Light Chains: Diagnostic and Prognostic Relevance in MS and CIS. PLoS ONE 2014;9:e89945
  4. Heaney JL, et al Salivary immunoglobulin free light chains: reference ranges and responses to exercise in young and older adults. Exerc Immunol Rev. 2016;22:28-41.
  5. Passerini, G, Free Light Chains and Intrathecal B Cells Activity in Multiple Sclerosis: A Prospective Study and Meta-Analysis. Multiple Sclerosis International Volume 2016, Article ID 2303857
  6. Ramsden, DB, Multiple sclerosis: assay of free immunoglobulin light chains. Annals of Clinical Biochemistry 2017, Vol. 54(1) 5–13
  7. Heaney JLJ, et al Intensified training increases salivary free light chains in trained cyclists: Indication that training volume increases oral inflammation. Physiol Behav. 2018 May 1;188:181-187. doi: 10.1016/j.physbeh.2018.02.013. Epub 2018 Feb 7.
  8. Jacob S, et al CSF Free Light Chain profile in Autoimmune Encephalitis. Presented at 13th International Congress of Neuroimmunology, ICC, Jerusalem, Israel September 26 – 29, 2016
  9. Spielmann G, et al The impact of long duration spaceflight on the function of plasma cells. Presented at ACSM Annual Meeting, Minneapolis, USA, 29th May – 2nd June 2018. Abstract and Poster