The following process describes how simple PCRD is to use.
Click to enlarge
Instructions on how to use PCRD can be found in the kit contents section above.
*Please note that operating protocols may differ between each method.
PCRD
A rapid alternative to DNA agarose gel electrophoresis
Abingdon Health manufactures nucleic acid lateral flow immunoassays (NALFIA), called PCRD, which can be used as an alternative to using DNA agarose gel electrophoresis. While taking only 10 minutes to detect the successful amplification of target DNA sequences PCRD is the choice for busy laboratories wanting fast results.
| Price per unit | |||||
| Code | Product | Format | 1 to 9 | 10 plus | |
| FD51673 | PCRD (box of 50 tests) | Cassette | £146.25 | £139.15 | Order online |
| FD51676 | PCRD FLEX (box of 50 tests) | Dipstick | £106.25 | £100.00 | Learn more |
PCRD kit contents
50 x PCRD Nucleic Acid Detection lateral flow assays.
5 x Extraction Buffer (10 mL)
1 x Instruction for use
If you have any questions about PCRD nucleic acid lateral flow assays please get in touch via our contact us page.
What is PCRD?
PCRD nucleic acid lateral flow assays are qualitative rapid assays designed to detect amplicons labelled with DIG/Biotin and/or FITC (or FAM)/Biotin, with the addition of a control line. The lateral flow test strips incorporate reagents which sequentially capture and allow the visualisation of double-stranded amplification products containing selected binding partners.
- A rapid, safe and sensitive alternative to ethidium bromide staining of agarose gels
- A simple method of amplicon detection that takes only 10 minutes
- Results are detectable by eye due to an aggregation of carbon particles at the capture lines
Advantages of nucleic acid detection with PCRD
In addition to being able to cope with large workloads, owing to their ease of use, the following benefits make PCRD an obvious choice for post DNA amplification nucleic acid confirmation.
- Safe to use – avoids using intercalating dye and UV light
- No special chemistry or equipment required
- Time to result of 10 minutes
- Easy to use
- High sensitivity
- Many strips can be run simultaneously
- Cost-effective
Compatible amplification methods
Compatible amplification methods
PCRD can be used in conjunction with the following amplification methods*:
- Polymerase Chain Reaction (PCR)
- Loop mediated isothermal amplification (LAMP)
- Recombinase Polymerase Amplification (RPA)
- Helicase dependent amplification (HDA)
Due to their versatility, our nucleic acid detection assays can be used in a number of applications. Examples include:
- Meat speciation testing
- Food and drink authenticity
- Rapid disease detection – veterinary, human and plant amongst others
Using lateral flow technology for nucleic acid detection
Read our article entitled, ‘How is lateral flow technology used for rapid nucleic acid detection?‘ to learn more about using NALFIA tests.
Online
PCRD can be bought online via Abingdon Health Group e-commerce website, www.pocketdiagnostic.com. Order online.
Bank transfer
For new customers and customers who prefer to order by bank transfer please send proforma requests, along with a company purchase order, to orders@abingdonhealth.com
Credit/debit card
We have the ability to take credit/debit card payments over the phone. To order by phone please call +44 (0) 1904 406080.
Quotation Request
If you would like to receive a quotation prior to ordering please request a quotation by clicking here.
PCRD is used by customers globally from many different industry sectors. Read our article about rapid nucleic acid detection to learn more.
Customisation of PCRD
Customisation of PCRD and PCRD FLEX is available upon request. Please contact a member of the team for more information.
Tel: +44 (0) 1904 406082
Email: info@abingdonhealth.com
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Learn more about PCRD and PCRD FLEX.
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