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Rapid alternative to DNA agarose gel electrophoresis
Abingdon Health manufactures nucleic acid lateral flow immunoassays (NALFIA), called PCRD, which can be used as an alternative to using DNA agarose gel electrophoresis. While taking only 10 minutes to detect the successful amplification of target DNA sequences PCRD is the choice for busy laboratories wanting fast results.
What is PCRD Nucleic Acid Detection?
PCRD Nucleic acid lateral flow assays are qualitative rapid assays designed to detect amplicons labelled with DIG/Biotin and/or FITC (or FAM)/Biotin, with the addition of a control line.
Detection of amplified DNA products using lateral flow assays
PCRD is a rapid, safe and sensitive way to confirm the successful outcome of a DNA amplification exercise. The lateral flow test strips incorporate reagents which sequentially capture and allow the visualisation of double-stranded amplification products containing selected binding partners.
This simple method of amplicon detection takes only 10 minutes, and the result is detectable by eye due to an aggregation of carbon particles at the capture lines.
Also, PCRD is a rapid, safe and sensitive alternative to ethidium bromide staining of agarose gels.
Advantages of using PCRD
In addition to being able to cope with large workloads, owing to their ease of use, the following benefits make PCRD an obvious choice for post DNA amplification nucleic acid confirmation.
- Safe to use – avoids using intercalating dye and UV light
- No special chemistry or equipment required
- Time to result of 10 minutes
- Easy to use
- High sensitivity
- Many strips can be run simultaneously
- Cost effective
Easy to use
The following process describes how simple PCRD is to use.
Click to enlarge
Instructions on how to use PCRD can be found in the kit contents section below.
Compatible amplification methods
Abingdon Health’s assays can be used in conjunction with the following amplification methods*:
*Please note that operating protocols may differ between each method.
- Polymerase Chain Reaction (PCR)
- Loop mediated isothermal amplification (LAMP)
- Recombinase Polymerase Amplification (RPA)
- Helicase dependent amplification (HDA).
Due to their versatility, our nucleic acid detection assays can be used in a number of applications. Examples include:
- Meat speciation testing
- Food and drink authenticity
- Rapid disease detection – veterinary, human and plant amongst others
How to order PCRD?
We have the ability to take orders through various means.
First of all, we have the ability to take credit/debit card payments over the phone. To order by phone please call +44 (0) 1904 406080.
For new customers and customers who prefer to order by bank transfer please send proforma requests, along with a company purchase order, to email@example.com
Ordering on account with credit is only available to eligible customers. For information about account ordering eligibility please contact us on firstname.lastname@example.org.
Finally, for organisations who would prefer to order PCRD online, we have set up a purchase page on Abingdon Health Group website e-commerce Pocket Diagnostic®. Click here to order online.
If you have any questions about PCRD nucleic acid lateral flow assays please get in touch via our contact us page.